Hosek Research Team
Hosek Research Team
Hocek Research Team

Mgr. et Mgr. Pavla Perlíková, Ph.D. (Spáčilová)

Mgr. et Mgr. Pavla Perlíková (Spáčilová)

e-mail: spacilova@uochb.cas.cz
phone: +420 220183343


Born 10. 3. 1984 in Ostrava (Czech Republic).

Scientific CV:
2003-2006 undergraduate study, Charles University in Prague - Faculty of Science, Bc. S. degree - specialization organic chemistry (supervisor Dr. Jan Šarek)
2004-2007 undergraduate study, Charles University in Prague - Faculty of Science, Bc. S. degree - specialization molecular biology and biochemistry (supervisor Associate Prof. Marián Hajdúch)
2006-2008 undergraduate study, Charles University in Prague - Faculty of Science, M. S. degree - specialization organic chemistry (supervisor RNDr. Jan Šarek, Ph.D.)
2007-2009 undergraduate study, Charles University in Prague - Faculty of Science, M. S. degree - specialization cell biology (supervisor Associate Prof. Marián Hajdúch)
2008-2012 PhD study, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (supervisor Dr. M. Hocek)
2012-2013 postdoctoral position, University of Southern Denmark (supervisor prof. Jesper Wengel)
since 2013 postdoctoral position, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (supervisor Dr. M. Hocek)

Professional membership:
Czech Chemical Society
IUPAC Affiliate Member

Awards:
2008 Zentiva award for the best diploma thesis (1st position)
2011 Best oral presentation nominee (section chemistry), XI Interdisciplinary Meeting of Young Biologists, Chemists and Biochemists, Žďár nad Sázavou
2011 Best oral presentation, XV Symposium on Chemistry of Nucleic Acid Components, Český Krumlov
2012 Prix Sanofi Pharmacie (3rd position)
2017 Otto Wichterle Award


List of publications:
20. Matyašovský, J.; Perlíková, P.; Malnuit, V.; Pohl, R.; Hocek, M.: "2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove" Angew. Chem. Int. Ed. 2016, 55, 15856 - 15859.
19. Bosáková, A.; Perlíková, P.; Tichý, M.; Pohl, R.; Hocek, M.: "6-Aryl-4-amino-pyrimido[4,5-b]indole 2'-deoxyribonucleoside triphosphates (benzo-fused 7-deaza-dATP analogues): Synthesis, fluorescent properties, enzymatic incorporation into DNA and DNA-protein binding study" Bioorg. Med. Chem. 2016, 24, 4528 - 4535.
18. Perlíková, P.; Rylová, G.; Nauš, P.; Elbert, T.; Tloušťová, E.; Bourderioux, A.; Poštová Slavětínská, L.; Motyka, K.; Doležal, D.; Znojek, P.; Nová, A.; Harvanová, M.; Džubák, P.; Šiller, M.; Hlaváč, J.; Hajdúch, M.; Hocek, M.: "7-(2-Thienyl)-7-Deazaadenosine (AB61), a New Potent Nucleoside Cytostatic with a Complex Mode of Action" Mol. Cancer Ther. 2016, 15, 922 – 937.
17. Raindlová, V.; Janoušková, M.; Slavíčková, M.; Perlíková, P.; Boháčová, S.; Milisavljevič, N.; Šanderová, H.; Benda, M.; Barvík, I.; Krásný, L.; Hocek, M.: "Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases" Nucleic Acids Res. 2016, 44, 3000 – 3012.
16. Güixens-Gallardo, P.; Hocek, M.; Perlíková, P.: "Inhibition of non-templated nucleotide addition by DNA polymerases in primer extension using twisted intercalating nucleic acid modified templates" Bioorg. Med. Chem. Lett. 2016, 26, 288 – 291.
15. Nauš, P.; Caletková, O.; Perlíková, P.; Slavětínská, L.; Tloušťová, E.; Hodek, J.; Weber, J.; Džubák, P.; Hajdúch, M.; Hocek, M.: "Synthesis and biological profiling of 6- or 7-(het)aryl-7-deazapurine 4'-C-methylribonucleosides" Bioorg. Med. Chem. 2015, 23, 7422 – 7438.
14. Mačková, M.; Boháčová, S.; Perlíková, P.; Poštová Slavětínská, L.; Hocek, M.: "Polymerase Synthesis and Restriction Enzyme Cleavage of DNA Containing 7-Substituted 7-Deazaguanine" ChemBioChem 2015, 16, 2225 – 2236.
13. Perlíková, P.; Ejlersen, M.; Langkjaer, N.; Wengel, J.: "Bis-Pyrene-Modified Unlocked Nucleic Acids: Synthesis, Hybridization Studies, and Fluorescent Properties" ChemMedChem 2014, 9, 2120 – 2127.
12. Nauš,P.; Caletková, O.; Konečný, P.; Džubák, P.; Bogdanová, K.; Kolář, M.; Vrbková, J.; Slavětínská, L.; Tloušťová, E.; Perlíková, P.; Hajdúch, M.; Hocek, M.: "Synthesis, Cytostatic, Antimicrobial, and Anti-HCV Activity of 6-Substituted 7-(Het)aryl-7-deazapurine Ribonucleosides" J. Med. Chem. 2014, 57, 1097 – 1110.
11. Perlíková, P.; Karlsen, K. K.; Pedersen, E. B.; Wengel, J.: "Unlocked Nucleic Acids with a Pyrene-Modified Uracil: Synthesis, Hybridization Studies, Fluorescent Properties and i-Motif Stability" ChemBioChem 2014, 15, 146 – 156.
10. Perlíková, P.; Konečný, P.; Nauš, P.; Snášel, J.; Votruba, I.; Džubák, P.; Pichová, I.; Hajdúch, M.; Hocek, M.: "6-Alkyl-, 6-aryl- or 6-hetaryl-7-deazapurine ribonucleosides as inhibitors of human or MTB adenosine kinase and potential antimycobacterial agents" Med. Chem. Commun. 2013, 4, 1497 – 1500.
9. Perlíková, P.; Eberlin, L.; Ménová, P.; Raindlová, V.; Slavětínská, L.; Tloušťová, E.; Bahador, G.; Lee, Y.-J.; Hocek, M.: "Synthesis and Cytostatic and Antiviral Activities of 2’-Deoxy-2’,2’-difluororibo- and 2’-Deoxy-2’-fluororibonucleosides Derived from 7-(Het)aryl-7-deazaadenines" ChemMedChem 2013, 8, 832-846.
8. Perlíková, P.; Jornet Martínez, N.; Slavětínská, L.; Hocek, M.: "Synthesis of 2'-deoxy-2'-fluororibo- and 2'-deoxy-2',2'-difluororibonucleosides derived from 6-(het)aryl-7-deazapurines" Tetrahedron 2012, 68, 8300-8310.
7. Nauš, P.; Perlíková, P.; Bourderioux, A.; Pohl, R.; Slavětínská, L.; Votruba, I.; Bahador, G.; Birkuš, G.; Cihlář, T.; Hocek, M.: "Sugar-modified derivatives of cytostatic 7-(het)aryl-7-deazaadenosines: 2'-C-methylribonucleosides, 2'-deoxy-2'-fluoroarabinonucleosides, arabinonucleosides and 2'-deoxyribonucleosides" Bioorg. Med. Chem. 2012, 20, 5202–5214.
6. Řezanka, M.; Řezanka, P.; Míka, L.; Perlíková, P.; Berka, K.: "Correspondence Seminar Inspired by Chemistry Topics (KSICHT)" Chem. Listy 2012, 106, 319-322.
5. Bourderioux, A.; Nauš, P.; Perlíková, P.; Pohl, R.; Pichová, I.; Votruba, I.; Džubák, P.; Konečný, P.; Hajdúch, M.; Stray, K.M.; Wang, T.; Ray, A.S.; Feng, J.Y.; Birkus, G.; Cihlar, T.; Hocek, M.: "Synthesis and Significant Cytostatic Activity of 7-Hetaryl-7-deazaadenosines" J. Med. Chem. 2011, 54, 5498–5507.
4. Nauš, P.; Perlíková, P.; Pohl, R.; Hocek, M.: "Sugar-modified derivatives of cytostatic 6-(het)aryl-7-deazapurine nucleosides: 2'-C-methylribonucleosides, arabinonucleosides and 2'-deoxy-2'-fluoroarabinonucleosides" Collect. Czech. Chem. Commun. 2011, 76, 957-988.
3. Perlíková, P.; Pohl, R.; Votruba, I.; Shih, R.; Birkuš, G.; Cihlář, T.; Hocek, M.: "Phosphoramidate pronucleotides of cytostatic 6-aryl-7-deazapurine ribonucleosides" Bioorg. Med. Chem. 2011, 19, 229-242.
2. Kvasnica, M.; Spacilova, P.; Budesinsky, M.; Hajduch, M.; Sarek, J.: "Oxidative Cleavage of 3beta-acetoxy-21-oxolup-18-en-28-oic Acid by Ruthenium Tetroxide" Chem. Nat. Compd. 2010, 46, 545-548.
1. Spáčilová, P.; Nauš, P.; Pohl, R.; Votruba, I.; Snášel, J.; Zábranská, H.; Pichová, I.; Ameral, R.; Birkuš, G.; Cihlář, T.; Hocek, M.: "CycloSal-phosphate Pronucleotides of Cytostatic 6-(Het)aryl-7-deazapurine Ribonucleosides: Synthesis, Cytostatic Activity, and Inhibition of Adenosine Kinases" ChemMedChem 2010, 5, 1386–1396.
Patents:
2. Šarek, J.; Spáčilová, P.; Hajduch, M.: "2-deoxyglykosidy triterpenoidů, způsob jejich přípravy a jejich použití jako léčivo" Patent Application PV 2008-732; 13th November 2008.
1. Šarek, J.; Hajduch, M.; Svoboda, M.; Nováková, K.; Spáčilová, P.; Kubelka, T.; Biedermann, D.: "Method of preparation of a soluble formulation of water-insoluble pentacyclic and tetracyclic terpenoids, a soluble formulation of a pentacyclic or tetracyclic terpenoid and a pharmaceutical composition containing this soluble formulation" Patent Application PCT/CZ2007/000088.
© Michal Hocek
Institute of Organic Chemistry and Biochemistry
Academy of Sciences of the Czech Republic