Hocek Research Team

RNDr. Marek Ondruš, Ph.D.

Marek Ondruš

e-mail: marek.ondrus@uochb.cas.cz

phone: +420 220 183 344, +420 725 910 615

Born 18. 02. 1992 in Žilina, Slovakia.

Scientific CV:

2011-2014 undergraduate study, Comenius University in Bratislava, Faculty of Science, Slovakia, B.Sc. Specialization in Biochemistry.

2014-2016 postgraduate study, Comenius University in Bratislava, Faculty of Science, Slovakia, M.Sc. Specialization in Organic and Bioorganic Chemistry.

2016-2023 Ph.D. Study, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (supervisor Prof. Hocek).

since 2023 Project leader, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (Prof. Hocek).

List of publications:

11.	Franco-Urquijo, P. A., Ondruš, M., Kurfürst, J., Škerlová, A., Selicharová, I., Mužíková Čechová, L., Šváchová, H., Semerádtová, A., Filimonĕnko, A., Fejfarová, A., Homola, J., Kouba, T., Hocek, M.: "Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries." Nat. Commun. 2026, https://doi.org/10.1038/s41467-026-73676-y.
10.	Ondruš, M.; Franco-Urquijo, P. A.; Lerga, T. M.; Mužíková Cechová, L.; Bermudo Redondo, M. C.; O´Sullivan, C. K. and Hocek, M.: "Versatile enzymatic approach for truncation, labeling, and mutation of nucleobase-modified aptamers demonstrated on indole-modified ß-conglutin aptamer" ChemBioChem 2025, 26(20), e202500534.
9.	Kaiser, L.; Ondruš, M.; Poštová Slavĕtínská, L.; Raindlová, V.; Hocek, M.: "Polymerase Synthesis of Hypermodified DNA Displaying a Combination of Thiol, Hydroxyl, Carboxylate, and Imidazole Functional Groups in the Major Groove" Chem. Eur. J. 2025, e202501034.
8.	Kuprikova, N.; Ondruš, M.; Bednárová, L.; Kraus, T.; Poštová Slavĕtínská, L.; Sýkorová, V.; Hocek, M.: "Zwitterionic DNA: enzymatic synthesis of hypermodified DNA bearing four different cationic substituents at all four nucleobases" Nucleic Acids Res. 2025, 53 (5), gkaf155.
7.	Ivancová, I.; Sanchez Quirante, T.; Ondruš, M.; Pohl, R.; Vlková, M.; Žilecká, E.; Bouřa, E. and Hocek, M.: "Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins" Commun. Chem. 2025, 8, 1.
6.	Kuprikova, N.; Ondruš, M.; Bednárová, L.; Riopedre Fernández, M.; Poštová Slavĕtínská, L.; Sýkorová, V.; Hocek, M.: "Superanionic DNA. Enzymatic Synthesis of Hypermodified DNA Bearing Four Different Anionic Substituents at all Four Nucleobases" Nucleic Acids Res. 2023, 51 (21), 11428-11438.
5.	Mulholland, C.; Jestřábová, I.; Sett, A.; Ondruš, M.; Sýkorová, V.; Manzanares, C. L.; Šimoncík, O.; Muller, P. and Hocek, M.: "The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70" Commun. Chem. 2023, 6, 65.
4.	Ondruš, M.; Sýkorová, V.; Hocek, M.: "Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates" Chem. Commun. 2022, 58, 11248-11251.
3.	Ondruš, M.; Sýkorová, V.; Bednárová, L.; Pohl, R.; Hocek, M.: "Enzymatic Synthesis of Hypermodified DNA Polymers for Sequence-Specific Display of Four Different Hydrophobic Groups" Nucleic Acids Res. 2020, 48 (21), 11982-11993.
2.	Dobiaš, J.; Ondruš, M.; Hlavác, M.; Murár, M.; Kóna, J.; Addová, G.; Bohác, A.: "Medicinal chemistry: an effect of a desolvation penalty of an amide group in the development of kinase inhibitors." Chem. Pap. 2019, 73, 71-84.
1.	Dobiaš, J.; Ondruš, M.; Addová, G.; Bohác, A.: "Switchable highly regioselective synthesis of 3,4-dihydroquinoxaline-2(1H)ones from o-phenylenediamines and aroylpyruvates." Beilstein J. Org. Chem. 2017, 13, 1350-1360.