Hosek Research Team
Hosek Research Team
Hocek Research Team

Ing. Veronika Sýkorová, Ph.D. (Raindlová)

Ing. Veronika Sýkorová

e-mail: veronika.sykorova@uochb.cas.cz
phone: +420 220183434


Born 15. 8. 1983 in Jeseník (Czech Republic).

Scientific CV:
2003-2008 undergraduate study, Prague Institute of Chemical Technology, M. S. degree - specialization organic chemistry (supervisor Ing. Miroslav Dudič, PhD.)
2008-2013 PhD study, Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (supervisor Dr. M. Hocek)
2013-2020 Postdoctoral stay (supervisor Dr. M. Hocek)
since 11/2020 Associate scientist (supervisor Dr. M. Hocek)

Career breaks:
4/2013-10/2014 1st Maternity Leave
4/2016-11/2017 2nd Maternity Leave

Grant projects:
2015-2017 Fellow researcher of standard project 15-08434S (Czech Science Foundation): Novel reactive groups introduced in nucleic acids for modification with oxoosmium complexes: structural and analytical aspects.

2022-2024 Fellow researcher of standard project 22-11252S (Czech Science Foundation): Electroactive and fluorescent modifications of nucleic acids for structure & interaction studies and for applications in diagnostics.


List of publications:
24. Kuprikova, N.; Ondruš, M.; Bednárová, L.; Riopedre Fernández, M.; Poštová Slavĕtínská, L.; Sýkorová, V.; Hocek, M.: "Superanionic DNA. Enzymatic Synthesis of Hypermodified DNA Bearing Four Different Anionic Substituents at all Four Nucleobases" Nucleic Acids Res. 2023, 51 (21), 11428-11438.
23. Kodr, D.; Ortiz, M.; Sýkorová, V.; Yenice, C. P.; Lesnikowski, Z. J.; O’Sullivan, C. K.; Hocek, M.: "Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition" Anal. Chem. 2023, 95, 12586-12589.
22. Mulholland, C.; Jestřábová, I.; Sett, A.; Ondruš, M.; Sýkorová, V.; Manzanares, C. L.; Šimoncík, O.; Muller, P. and Hocek, M.: "The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70" Commun. Chem. 2023, 6, 65.
21. Spampinato, A.; Kužmová, E.; Pohl, R.; Sýkorová, V.; Vrábel, M.; Kraus, T. and Hocek, M.: "trans-Cyclooctene- and Bicyclononyne-Linked Nucleotides for Click Modification of DNA with Fluorogenic Tetrazines and Live Cell Metabolic Labeling and Imaging" Bioconjugate Chem. 2023, 34(4), 772-780.
20. Ondruš, M.; Sýkorová, V.; Hocek, M.: "Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates" Chem. Commun. 2022, 58, 11248-11251.
19. Pospíšil, Š.; Panattoni, A.; Gracias, F.; Sýkorová, V.; Hausnerová, V. V.; Vítovská, D.; Šanderová, H.; Krásný, L.; Hocek, M.: "Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases" ACS Chem. Biol. 2022, 17 (10), 2781-2788.
18. Gracias, F.; Ruiz-Larrabeiti, O.; Vanková Hausnerová, V.; Pohl, R.; Klepetárová, B.; Sýkorová, V.; Krásný, L.; Hocek, M.: "Homologues of epigenetic pyrimidines: 5-alkyl-, 5-hydroxyalkyl and 5-acyluracil and -cytosine nucleotides: synthesis, enzymatic incorporation into DNA and effect on transcription with bacterial RNA polymerase" RSC Chem. Biol. 2022, 3, 1069-1075.
17. Leone, D.-L.; Pohl, R.; Hubálek, M.; Kaderábková, M.; Krömer, M.; Sýkorová, V.; Hocek, M.: "Glyoxal-Linked Nucleotides and DNA for Bioconjugations and Cross-Linking with Arginine-Containing Peptides and Proteins" Chem. Eur. J. 2022, 28, e202104208.
16. Sýkorová, V.; Tichý, M.; Hocek, M.: "Polymerase Synthesis of DNA Containing Iodinated Pyrimidine or 7-Deazapurine Nucleobases and Their Post-synthetic Modifications through the Suzuki-Miyaura Cross-Coupling Reactions" ChemBioChem 2022, 23, e202100608.
15. Kodr, D.; Yenice, C. P.; Simonova, A.; Saftic, D. P.; Pohl, R.; Sýkorová, V.; Ortiz, M.; Havran, L.; Fojta, M.; Lesnikowski, Z. J.; O’Sullivan, C. K.; Hocek, M.: "Carborane- or Metallacarborane-Linked Nucleotides for Redox Labeling. Orthogonal Multipotential Coding of all Four DNA Bases for Electrochemical Analysis and Sequencing" J. Am. Chem. Soc. 2021, 143 (18), 7124-7134.
14. Milisavljevic, N.; Konkolová, E.; Kozák, J.; Hodek, J.; Veselovská, L.; Sýkorová, V.; Cížek, K.; Pohl, R.; Eyer, L.; Svoboda, P.; Ružek, D.; Weber, J.; Nencka, R.; Boura, E.; Hocek, M.: "Antiviral Activity of 7-Substituted 7-Deazapurine Ribonucleosides, Monophosphate Prodrugs, and Triphoshates against Emerging RNA Viruses" ACS Infect. Dis. 2021, 7 (2), 471-478.
13. Ondruš, M.; Sýkorová, V.; Bednárová, L.; Pohl, R.; Hocek, M.: "Enzymatic Synthesis of Hypermodified DNA Polymers for Sequence-Specific Display of Four Different Hydrophobic Groups" Nucleic Acids Res. 2020, 48 (21), 11982-11993.
12. Simonova, A.; Magriná, I.; Sýkorová, V.; Pohl, R.; Ortiz, M.; Havran, L.; Fojta, M.; O'Sullivan, C. K.; Hocek, M.: "Tuning of Oxidation Potential of Ferrocene for Ratiometric Redox Labeling and Coding of Nucleotides and DNA" Chem. Eur. J. 2020, 26, 1286-1291.
11. Havranová-Vidláková, P.; Krömer, M.; Sýkorová, V.; Trefulka, M.; Fojta, M.; Havran, L.; Hocek, M.: "Vicinal Diol-Tethered Nucleobases as Targets for DNA Redox Labeling with Osmate Complexes" ChemBioChem 2020, 21, 171-180.
10. Krömer, M.; Bártová, K.; Raindlová, V.; Hocek, M.: "Synthesis of dihydroxyalkynyl and dihydroxyalkyl nucleotides as building blocks or precursors for introduction of diol or aldehyde functions to DNA for bioconjugations" Chem. Eur. J. 2018, 24, 11890-11894.
9. Havranová-Vidláková, P.; Špaček, J.; Vítová, L.; Hermanová, M.; Daďová, J.; Raindlová, V.; Hocek, M.; Fojta, M.; Havran, L.: "Butylacrylate-nucleobase Conjugates as Targets for Two-step Redox Labeling of DNA with an Osmium Tetroxide Complex" Electroanalysis 2018, 30, 371 - 377.
8. Raindlová, V.; Janoušková, M.; Slavíčková, M.; Perlíková, P.; Boháčová, S.; Milisavljevič, N.; Šanderová, H.; Benda, M.; Barvík, I.; Krásný, L.; Hocek, M.: "Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases" Nucleic Acids Res. 2016, 44, 3000 – 3012.
7. Danhel, A.; Raindlova, V.; Havran, L.; Barek, J.; Hocek, M.; Fojta, M.: "Voltammetric Study of dsDNA Modified by Multi-redox Label Based on N-methyl-4-hydrazino-7-nitrobenzofurazan" Electrochimica Acta 2014, 129, 348 - 357.
6. Danhel, A.; Raindlova, V.; Havran, L.; Pivonkova, H.; Hocek, M.; Fojta, M.: "Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne as multi-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode" Electrochimica Acta 2014, 126, 122 - 131.
5. Ménová, P.; Raindlová, V.; Hocek, M.: "Scope and Limitations of the Nicking Enzyme Amplification Reaction for the Synthesis of Base-Modified Oligonucleotides and Primers for PCR" Bioconjugate Chem. 2013, 24, 1081-1093.
4. Perlíková, P.; Eberlin, L.; Ménová, P.; Raindlová, V.; Slavětínská, L.; Tloušťová, E.; Bahador, G.; Lee, Y.-J.; Hocek, M.: "Synthesis and Cytostatic and Antiviral Activities of 2’-Deoxy-2’,2’-difluororibo- and 2’-Deoxy-2’-fluororibonucleosides Derived from 7-(Het)aryl-7-deazaadenines" ChemMedChem 2013, 8, 832-846.
3. Raindlová, V.; Pohl, R.; Klepetářová, B.; Havran, L.; Šimková, E.; Horáková, P.; Pivoňková, H.; Fojta, M.; Hocek, M.: "Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling" ChemPlusChem 2012, 77, 652–662.
2. Raindlová, V.; Pohl, R.; Hocek, M.: "Synthesis of Aldehyde-Linked Nucleotides and DNA and Their Bioconjugations with Lysine and Peptides through Reductive Amination" Chem. Eur. J. 2012, 18, 4080-4087.
1. Raindlová, V; Pohl, R.; Šanda, M.; Hocek, M.: "Direct Polymerase Synthesis of Reactive Aldehyde-Functionalized DNA and Its Conjugation and Staining with Hydrazines" Angew. Chem. Int. Ed. 2010, 49, 1064–1066.
© Michal Hocek
Institute of Organic Chemistry and Biochemistry
Academy of Sciences of the Czech Republic