Novel types of modified derivatives and analogues of nucleobases, nucleosides, nucleotides and nucleic acids are designed and prepared for applications in all areas of biomedicinal sciences (medicinal chemistry, biochemistry, chemical biology, bioanalysis etc.).
Basic developments of methodology for the synthesis of these modified biomolecules are performed largely using modern methods (including metal- or enzyme-catalyzed reactions). Biological activity (cytostatic, antiviral etc.) of the novel nucleobases, nucleosides and nucleotides is systematically studied in collaboration with several groups both in academia and in pharmaceutical industry. Several collaborative projects also address chemical biology of nucleic acids (i.e. DNA polymerase specificity and fidelity). Functionalized nucleic acids bearing diverse interesting and useful substituents are another goal of our group. For details, see Projects.
Kuba, M. et. al: "Real-time Imaging of Nascent DNA in Live Cells by Monitoring the Fluorescence Lifetime of DNA-Incorporated Thiazole Orange-Modified Nucleotides" Angew. Chem. Int. Ed. 2023, 62, e202307548.
Mulholland, C. et. al: "The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70" Commun. Chem. 2023, 6, 65.